Journal: Journal of Extracellular Biology
Article Title: Workflow for the Isolation and Characterisation of Human Milk Extracellular Vesicles (HMEVs) and Their Inflammatory Biomarker Profile
doi: 10.1002/jex2.70110
Figure Lengend Snippet: Evaluation of different beta‐casein removal approaches. Beta‐casein bands from Western blot analysis (top) and total protein content determined by ATR‐FTIR (bottom). Control = skimmed HM. Error bars indicate standard deviation ( N = 3). ATR‐FTIR, attenuated total reflectance–Fourier transform infrared; CaCl 2 , calcium chloride; HAc, acetic acid; HCl, hydrochloric acid; KH 2 PO 4 , potassium dihydrogen phosphate.
Article Snippet: Primary antibodies against CD81, Alix, β‐casein and secondary antibody against IgG were purchased from Santa Cruz Biotech (USA).
Techniques: Western Blot, Control, Standard Deviation, Fourier Transform Infrared Spectroscopy